Day 1

• Introduction to protein assignment and structure calculation:
  
  • General strategy
  • Sequential assignment
  • Side chain assignment
• Introduction to CCPN Analysis:
  • Core concepts
  • Navigation and basic commands
  • Setting up the course project
  • Customising the project

Day 2

• 2D 15N-HSQC peak picking
• Assigning root resonances
• 3D peak picking based on 2D root peaks
• Assigning non root resonances
• Identifying spin system types
• Navigating in hCCH-Tocsy spectra
• Assigning side chain resonances to atom types

Day 3

• Manual sequential assignment
• Aromatic side chain assignment
• Completion of side chain assignment

Day 4

• Mapping assignments to NOESY spectra
• Peak picking of NOESY spectra
• Preparing data for structure calculation
• Setting up and running a structure calculation with CYANA

Day 5

• Evaluation of the structure calculations
• Introduction to the YASARA structure viewer: Structure inspection
• General evaluation and questions